DNA Delivery Redesign Doubles Plant Transformations and Boosts Fungal Edits by 400%

Karmactive Staff Karmactive Staff

Berkeley Lab engineers boost plant DNA transfer success by 100% while fungal modifications surge 400% through bacterial vector tweaks.

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[{"selector":"#anim-01b72a18-34f7-4d77-ac12-a2657d183b9a","keyframes":{"opacity":[0,1]},"delay":500,"duration":2000,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-7df4eacb-7a31-46c1-bf01-15bcff6622c3","keyframes":{"opacity":[0,1]},"delay":1000,"duration":2000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] [{"selector":"#anim-83b46ab3-55af-44c9-bd42-e4a8e74a5a63","keyframes":{"transform":["translate3d(0px, 251.11070%, 0)","translate3d(0px, 0px, 0)"]},"delay":1000,"duration":2000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] [{"selector":"#anim-05536c51-6fd7-4141-af13-0a75e3a22ea6 [data-leaf-element=\"true\"]","keyframes":{"transform":["translate3d(31.357977989224374%, 0, 0)","translate3d(0%, 0, 0)"]},"delay":0,"duration":2000,"easing":"cubic-bezier(.3,0,.55,1)","fill":"both"}] How will modified binary vectors revolutionize 40-year-old plant transformation techniques? Photo Source: Alliance of Biove (CC BY-NC-SA 2.0) Photo Source: Alliance of Biove (CC BY-NC-SA 2.0)

What makes scientists target origin of replication for increasing plasmid copy numbers?

[{"selector":"#anim-aec9367c-8758-4e43-bec9-99f3197e98a9","keyframes":{"transform":["translate3d(-92.2619%, 0px, 0)","translate3d(0px, 0px, 0)"]},"delay":0,"duration":2000,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-a3330f5a-47c8-4ec8-9afa-2010a81fc839","keyframes":{"opacity":[0,1]},"delay":0,"duration":2000,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-79e35bde-8f62-47c3-a0a9-6d87e186a6ee","keyframes":{"transform":["scale(0.15)","scale(1)"]},"delay":0,"duration":2000,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"forwards"}] [{"selector":"#anim-ee2b7a34-3233-49d5-9598-7b8eb40bceb0","keyframes":{"transform":["translate3d(115.18987%, 0px, 0)","translate3d(0px, 0px, 0)"]},"delay":500,"duration":1500,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-142d898e-10ce-4360-b9f8-09c5a2b51733","keyframes":{"opacity":[0,1]},"delay":500,"duration":1500,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-35d637dc-4352-47df-9636-5e36cbedda81","keyframes":{"transform":["scale(0.15)","scale(1)"]},"delay":500,"duration":1500,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"forwards"}] [{"selector":"#anim-0a4046ef-a190-4829-bab5-3e83e9debb39","keyframes":{"opacity":[0,1]},"delay":1000,"duration":2000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] [{"selector":"#anim-03ca7a33-1cfb-4e33-8428-3e9fd38dae99","keyframes":{"transform":["translate3d(0px, 251.11070%, 0)","translate3d(0px, 0px, 0)"]},"delay":1000,"duration":2000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] Photo Source: pixabay Photo Source: pixabay

[{"selector":"#anim-59c09612-42c6-4ce4-baad-63f523250c1f","keyframes":{"opacity":[0,1]},"delay":500,"duration":2000,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-56cdd8ec-e3ba-4ddd-881f-a43a9e5fe320","keyframes":{"opacity":[0,1]},"delay":1000,"duration":2000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] [{"selector":"#anim-e824b8f3-8bbc-4899-80a4-fb258893004a","keyframes":{"transform":["translate3d(0px, 251.11070%, 0)","translate3d(0px, 0px, 0)"]},"delay":1000,"duration":2000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] [{"selector":"#anim-df814a7c-f681-4dbf-a0e2-21d785cd439b [data-leaf-element=\"true\"]","keyframes":{"transform":["translate3d(31.233358876481383%, 0, 0)","translate3d(0%, 0, 0)"]},"delay":0,"duration":2000,"easing":"cubic-bezier(.3,0,.55,1)","fill":"both"}] Lab tests reveal varied transformation success through red reporter pigments in Arabidopsis plants. Photo Source: SozvezdieL (CC BY 4.0) Photo Source: SozvezdieL (CC BY 4.0)

CRISPR-Cas9 delivery improves as engineered vectors enhance genetic modification capabilities.

[{"selector":"#anim-f1fea2e7-4d12-4b96-867e-ec95332b25b7","keyframes":{"transform":["translate3d(115.18987%, 0px, 0)","translate3d(0px, 0px, 0)"]},"delay":500,"duration":1500,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-07c36a0c-320b-45a4-87fe-920e098c880f","keyframes":{"opacity":[0,1]},"delay":500,"duration":1500,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-97b307fa-0b8f-4486-967f-580361c73756","keyframes":{"transform":["scale(0.15)","scale(1)"]},"delay":500,"duration":1500,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"forwards"}] [{"selector":"#anim-28b4bce8-98d7-4390-90a2-05466acd2124","keyframes":{"transform":["translate3d(-92.2619%, 0px, 0)","translate3d(0px, 0px, 0)"]},"delay":0,"duration":3000,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-8843a721-6f8a-4d0d-b9dc-f4f4082178a4","keyframes":{"opacity":[0,1]},"delay":0,"duration":3000,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-616e362d-c1ae-4c97-a078-dcd98d2ba6ee","keyframes":{"transform":["scale(0.15)","scale(1)"]},"delay":0,"duration":3000,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"forwards"}] [{"selector":"#anim-03294cf2-1d23-4a6a-9655-ccac81adc98e","keyframes":{"opacity":[0,1]},"delay":1000,"duration":2000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] [{"selector":"#anim-7d3417ba-b8e5-4275-8c3d-85030f296c37","keyframes":{"transform":["translate3d(0px, 251.11070%, 0)","translate3d(0px, 0px, 0)"]},"delay":1000,"duration":2000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] Photo Source: IAEA Imagebank (CC BY-SA 2.0) Photo Source: IAEA Imagebank (CC BY-SA 2.0)

[{"selector":"#anim-ab997d70-8e4d-4ad4-b945-b96f05dff82f","keyframes":{"opacity":[0,1]},"delay":500,"duration":2000,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-62975180-b120-4d82-a6a0-4c7b1889abcf","keyframes":{"opacity":[0,1]},"delay":1000,"duration":2000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] [{"selector":"#anim-bf566cae-c82a-4794-8d94-e8b4b51d034a","keyframes":{"transform":["translate3d(0px, 251.11070%, 0)","translate3d(0px, 0px, 0)"]},"delay":1000,"duration":2000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] [{"selector":"#anim-a9847948-1c91-4114-adf1-e925b766350d [data-leaf-element=\"true\"]","keyframes":{"transform":["translate3d(31.22511913033763%, 0, 0)","translate3d(0%, 0, 0)"]},"delay":0,"duration":2000,"easing":"cubic-bezier(.3,0,.55,1)","fill":"both"}] Scientists conduct directed evolution assays across four AMT origins to select higher copy mutations. Photo Source: U.S. Department (PDM 1.0) Photo Source: U.S. Department (PDM 1.0)

Nature Biotechnology publishes findings as researchers target fossil fuel dependency reduction.

[{"selector":"#anim-36fbab47-e396-4389-a866-b77cb5cf2f4e","keyframes":{"transform":["translate3d(115.50632%, 0px, 0)","translate3d(0px, 0px, 0)"]},"delay":500,"duration":1500,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-92ec14d9-d0a5-4d53-b835-276781ea8a43","keyframes":{"opacity":[0,1]},"delay":500,"duration":1500,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-f0191ac5-0cd8-48a3-9e39-5735f7d888b7","keyframes":{"transform":["scale(0.15)","scale(1)"]},"delay":500,"duration":1500,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"forwards"}] [{"selector":"#anim-8a329032-fdc7-4f2f-8986-5c55d7537077","keyframes":{"transform":["translate3d(-92.2619%, 0px, 0)","translate3d(0px, 0px, 0)"]},"delay":0,"duration":2000,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-ba119a1d-5a9d-4baa-bdfe-0dac87abe462","keyframes":{"opacity":[0,1]},"delay":0,"duration":2000,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-71d38ac0-8834-4eea-8817-992dc3e62457","keyframes":{"transform":["scale(0.15)","scale(1)"]},"delay":0,"duration":2000,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"forwards"}] [{"selector":"#anim-5633d634-12dc-423e-bf3a-fce207d6a8d9","keyframes":{"opacity":[0,1]},"delay":1000,"duration":2000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] [{"selector":"#anim-a307184f-9c5a-49fe-b7c1-fe566fb8f888","keyframes":{"transform":["translate3d(0px, 256.11093%, 0)","translate3d(0px, 0px, 0)"]},"delay":1000,"duration":2000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] Photo Source: Sophie Winter (CC BY-NC 3.0 US) Photo Source: Sophie Winter (CC BY-NC 3.0 US)

[{"selector":"#anim-e08f64a6-fdef-4405-acf6-29dd6438c791","keyframes":{"opacity":[0,1]},"delay":500,"duration":2000,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-ece8539e-c25d-41a1-b6f8-7611522d6106","keyframes":{"opacity":[0,1]},"delay":1000,"duration":2000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] [{"selector":"#anim-ccea2ad3-9f0f-482b-96d7-b80f52316df2","keyframes":{"transform":["translate3d(0px, 224.86772%, 0)","translate3d(0px, 0px, 0)"]},"delay":1000,"duration":2000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] [{"selector":"#anim-75b3dd58-b7fd-4e84-9aea-250b55807e93 [data-leaf-element=\"true\"]","keyframes":{"transform":["translate3d(28.904257299848474%, 0, 0)","translate3d(0%, 0, 0)"]},"delay":0,"duration":2000,"easing":"cubic-bezier(.3,0,.55,1)","fill":"both"}] Berkeley graduate student highlights decades of unchanged vector structure use in plant science. Photo Source: Pacific Southwe (CC BY 2.0) Photo Source: Pacific Southwe (CC BY 2.0)

Modified Agrobacterium shows promise for pharmaceutical and biomaterial fungal transformations.

[{"selector":"#anim-e135357f-1c63-4f5b-9ac8-177b56aa1919","keyframes":{"transform":["translate3d(115.50632%, 0px, 0)","translate3d(0px, 0px, 0)"]},"delay":500,"duration":1500,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-81fd8f01-488c-413c-b6d3-7c3471440546","keyframes":{"opacity":[0,1]},"delay":500,"duration":1500,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-1f3b9970-4c96-4bc0-8840-7dd69cb94823","keyframes":{"transform":["scale(0.15)","scale(1)"]},"delay":500,"duration":1500,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"forwards"}] [{"selector":"#anim-9e37cef3-71f1-4858-83b8-4c4cd013a143","keyframes":{"transform":["translate3d(-92.44533%, 0px, 0)","translate3d(0px, 0px, 0)"]},"delay":0,"duration":2000,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-55d4ec15-5082-42cc-82fc-607a4cd3901d","keyframes":{"opacity":[0,1]},"delay":0,"duration":2000,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-8f151982-b7eb-4e61-a2b3-d05051f185d8","keyframes":{"transform":["scale(0.15)","scale(1)"]},"delay":0,"duration":2000,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"forwards"}] [{"selector":"#anim-ea34ae6e-6844-4472-8dab-b0aa274c520d","keyframes":{"opacity":[0,1]},"delay":1000,"duration":2000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] [{"selector":"#anim-26cdaed4-135e-4e82-a8dc-f8cf70f09d2b","keyframes":{"transform":["translate3d(0px, 256.11093%, 0)","translate3d(0px, 0px, 0)"]},"delay":1000,"duration":2000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] Photo Source: U.S. Departmen (CC BY 2.0) Photo Source: U.S. Departmen (CC BY 2.0)

[{"selector":"#anim-26ed633b-722a-4f7f-bbba-eb9185c7eec7","keyframes":{"opacity":[0,1]},"delay":0,"duration":1000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] [{"selector":"#anim-9224dbe0-37fd-4766-a1b0-1da90a0845cd","keyframes":{"transform":["translate3d(0px, -207.96649%, 0)","translate3d(0px, 0px, 0)"]},"delay":0,"duration":1000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] [{"selector":"#anim-2f01e9a9-ccd8-472f-afa9-9f497615f557","keyframes":{"opacity":[0,1]},"delay":0,"duration":1000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] [{"selector":"#anim-c0f9b927-1152-40ca-84f4-74b5c21d4997","keyframes":{"transform":["translate3d(-114.87342%, 0px, 0)","translate3d(0px, 0px, 0)"]},"delay":0,"duration":1000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] [{"selector":"#anim-2a0cdb35-5735-4083-888f-a3594f93c26b","keyframes":{"opacity":[0,1]},"delay":0,"duration":1000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] [{"selector":"#anim-58a8e2de-1b59-45f8-99c5-6b0f41ff75a6","keyframes":{"transform":["rotate(-360deg) translate3d(115.14195%, 0px, 0) rotate(360deg)","rotate(-360deg) translate3d(0px, 0px, 0) rotate(360deg)"]},"delay":0,"duration":1000,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] Daytime Cooling in Urban Green Spaces Offset by Increased Humidity Learn more